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primescripttm rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm rt reagent kit
    Primescripttm Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 89259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+kit/PrimeScript+RT+Reagent+Kit/10__17221_slash_9_slash_2026___cjas-76-11-18
    Average 99 stars, based on 89259 article reviews
    primescripttm rt reagent kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Benzoylaconitine and ginsenoside Rb1 synergistically attenuate cardiac remodeling through dual enhancement of DLG1-dependent mitochondrial integrity.
    Article Snippet: Background: The synergistic cardioprotective effects of monoester alkaloids and ginsenosides constitute the pharmacological basis of Shenfu decoction (SFD) for chronic heart failure.. However, the underlying molecular interactions remain unclear.. Purpose: This study aimed to identify the key bioactive components of SFD, elucidate their molecular targets, and define the mechanistic pathways underlying their synergistic cardioprotective effects.

    Article Title: Comprehensive analysis suggests CRIF1 is a potential target in breast cancer associated with prognosis and immune infiltration
    Article Snippet: Total RNA was isolated from tissues with Trizol (Invitrogen) and quantified with the Nanodrop 2000 (ThermoFisher Scientific). .. The PrimeScriptTM RT kit and gDNA Eraser (TaKaRa) were used for reverse transcription. ..

    Article Title: Chiral Fe 3 O 4 /GelMA hydrogels regulate the osteoimmune microenvironment via Itgb3-mediated macrophage polarization to combat peri-implantitis
    Article Snippet: The reverse transcription step was performed using a PCR thermocycler (Takara). .. The mRNA was reverse transcribed into cDNA using a PrimeScriptTM RT kit (Takara Bio, Otsu, Japan). cDNA samples were amplified by qRT-PCR according to the primer sequences listed below and normalized to the housekeeping gene β-actin. ..

    Article Title: The inhibitors of miR-224-5p, miR-339-5p, and miR-1198-5p improve auditory function by promoting the expression of neuritin in hearing loss mice
    Article Snippet: To detect miRNAs expression, total RNA was extracted from the organ of Corti using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA samples were reverse-transcribed using the PrimeScriptTM RT Kit (Takara Bio, Shiga, Japan). .. All primers used in this study were synthesized by RiboBio Co., Ltd. (Guangzhou, China, ).

    Article Title: Butyrate-producing gut bacteria restrain PBAT microplastic-triggered brain microglial lipotoxicity via a microbiota-butyrate-mTORC1-ISR relay along the gut-brain axis.
    Article Snippet: .. Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted with TRIzol reagent (Ambion, Austin, TX, USA) and reverse-transcribed using PrimeScriptTM RT kit (TaKaRa, Shiga, Japan). qRT-PCR was performed using primers listed in Supplementary Table S3. ..

    Article Title: MYC promotes myocardial fibrosis via METTL1-mediated m7G modification of HILPDA.
    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cardiac tissues and MCFs using TRIzol reagent (Servicebio), and its concentration and purity were AR TIC LE IN PR ES S determined using a spectrophotometer. .. Subsequently, 1 μg of total RNA from each sample was reverse-transcribed into cDNA using the PrimeScriptTM RT Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was then performed in triplicate using TB Green® Premix Ex TaqTM II Kit (Takara) on a realtime PCR detection system. ..

    Article Title: Identification of biomarkers associated with pathological dynamic progression in dystrophinopathies.
    Article Snippet: Following centrifugation, the upper aqueous phase was collected and mixed with isopropanol (Sigma-Aldrich, Cat#11335) at a 1:1 volume ratio to precipitate total RNA. .. RNA (1 μg) was reverse-transcribed into cDNA using the PrimeScriptTM RT kit with gDNA Eraser (Takara Bio Inc., Cat#RR047), according to the manufacturer’s instructions. .. To detect core gene mutations in wild-type and mdx mice, qPCR was performed using the cDNA with TB Green Premix Ex TaqTM (Takara Bio Inc., Cat#RR420A;).

    Real-time Polymerase Chain Reaction:

    Article Title: Benzoylaconitine and ginsenoside Rb1 synergistically attenuate cardiac remodeling through dual enhancement of DLG1-dependent mitochondrial integrity.
    Article Snippet: Background: The synergistic cardioprotective effects of monoester alkaloids and ginsenosides constitute the pharmacological basis of Shenfu decoction (SFD) for chronic heart failure.. However, the underlying molecular interactions remain unclear.. Purpose: This study aimed to identify the key bioactive components of SFD, elucidate their molecular targets, and define the mechanistic pathways underlying their synergistic cardioprotective effects.

    Article Title: Butyrate-producing gut bacteria restrain PBAT microplastic-triggered brain microglial lipotoxicity via a microbiota-butyrate-mTORC1-ISR relay along the gut-brain axis.
    Article Snippet: .. Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted with TRIzol reagent (Ambion, Austin, TX, USA) and reverse-transcribed using PrimeScriptTM RT kit (TaKaRa, Shiga, Japan). qRT-PCR was performed using primers listed in Supplementary Table S3. ..

    Synthesized:

    Article Title: A single-cell transcriptomic atlas identifies key progenitor populations driving postnatal horn bud development in goats (Capra hircus).
    Article Snippet: Total RNA was then extracted using RNAiso Plus (Takara, China) following the manufacturer’s protocol. .. Subsequently, cDNA was synthesized from 1 μg of RNA from each sample using the PrimeScriptTM RT kit (Takara, China). .. A quantitative real-time PCR assay quantified gene expression using SYBR Premix Ex TaqTM II (Takara, China).

    Amplification:

    Article Title: Chiral Fe 3 O 4 /GelMA hydrogels regulate the osteoimmune microenvironment via Itgb3-mediated macrophage polarization to combat peri-implantitis
    Article Snippet: The reverse transcription step was performed using a PCR thermocycler (Takara). .. The mRNA was reverse transcribed into cDNA using a PrimeScriptTM RT kit (Takara Bio, Otsu, Japan). cDNA samples were amplified by qRT-PCR according to the primer sequences listed below and normalized to the housekeeping gene β-actin. ..

    Quantitative RT-PCR:

    Article Title: Chiral Fe 3 O 4 /GelMA hydrogels regulate the osteoimmune microenvironment via Itgb3-mediated macrophage polarization to combat peri-implantitis
    Article Snippet: The reverse transcription step was performed using a PCR thermocycler (Takara). .. The mRNA was reverse transcribed into cDNA using a PrimeScriptTM RT kit (Takara Bio, Otsu, Japan). cDNA samples were amplified by qRT-PCR according to the primer sequences listed below and normalized to the housekeeping gene β-actin. ..

    Article Title: Butyrate-producing gut bacteria restrain PBAT microplastic-triggered brain microglial lipotoxicity via a microbiota-butyrate-mTORC1-ISR relay along the gut-brain axis.
    Article Snippet: .. Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted with TRIzol reagent (Ambion, Austin, TX, USA) and reverse-transcribed using PrimeScriptTM RT kit (TaKaRa, Shiga, Japan). qRT-PCR was performed using primers listed in Supplementary Table S3. ..

    Article Title: MYC promotes myocardial fibrosis via METTL1-mediated m7G modification of HILPDA.
    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cardiac tissues and MCFs using TRIzol reagent (Servicebio), and its concentration and purity were AR TIC LE IN PR ES S determined using a spectrophotometer. .. Subsequently, 1 μg of total RNA from each sample was reverse-transcribed into cDNA using the PrimeScriptTM RT Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was then performed in triplicate using TB Green® Premix Ex TaqTM II Kit (Takara) on a realtime PCR detection system. ..

    Polymerase Chain Reaction:

    Article Title: MYC promotes myocardial fibrosis via METTL1-mediated m7G modification of HILPDA.
    Article Snippet: Quantitative real-time PCR (qRT-PCR) Total RNA was extracted from cardiac tissues and MCFs using TRIzol reagent (Servicebio), and its concentration and purity were AR TIC LE IN PR ES S determined using a spectrophotometer. .. Subsequently, 1 μg of total RNA from each sample was reverse-transcribed into cDNA using the PrimeScriptTM RT Kit (Takara, Japan) according to the manufacturer's instructions. qRT-PCR was then performed in triplicate using TB Green® Premix Ex TaqTM II Kit (Takara) on a realtime PCR detection system. ..



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